| 1 | 21st Sep | 2:00pm | Gloucester Ladies Jades | 9 | v | 59 | Hucclecote Tornadoes | OxstallS Arena Ct1 | |
| 2 | 28th Sep | 12:30pm | Gloucester Ladies Jades | Conc | v | Won | Steelers Inferno | Leisure@ Ct1 | |
| 3 | 5th Oct | 2:00pm | Gloucester Ladies Jades | 26 | v | 39 | Wildcats Panthers | Leisure@ Ct1 | |
| 4 | 19th Oct | 9:30am | Gloucester Ladies Jades | 20 | v | 52 | Cheltenham Ladies Phoenix | Leisure@ Ct1 | |
| 5 | 26th Oct | 2:00pm | Gloucester Ladies Jades | Conc | v | Won | Steelers Embers | Leisure@ Ct1 | |
| 6 | 16th Nov | 12:30pm | Gloucester Ladies Jades | 23 | v | 57 | Moody Blues | Leisure@ Ct1 | |
| 7 | 23rd Nov | 11:00am | Gloucester Ladies Jades | 23 | v | 43 | Forest Foxes | Leisure@ Ct1 | |
| 8 | 30th Nov | 11:30am | Gloucester Ladies Jades | Conc | v | Won | Seven Up B | High School Leckhampton | |
| 9 | 7th Dec | 11:30am | Gloucester Ladies Jades | 37 | v | 34 | Challengers Galaxy | High School Leckhampton | |
| 10 | 14th Dec | 11:30am | Gloucester Ladies Jades | 26 | v | 72 | Gloucester City Queens Academy | High School Leckhampton | |
| 11 | 11th Jan | 2:00pm | Gloucester Ladies Jades | Won | v | Conc | Cirencester Aqua | Leisure@ Ct1 | |
| 12 | 18th Jan | 2:00pm | Gloucester Ladies Jades | 13 | v | 44 | Hucclecote Tornadoes | Leisure@ Ct1 | |
| 13 | 25th Jan | 12:30pm | Gloucester Ladies Jades | 30 | v | 39 | Steelers Inferno | Leisure@ Ct1 | |
| 14 | 1st Feb | 2:00pm | Gloucester Ladies Jades | 24 | v | 34 | Wildcats Panthers | Leisure@ Ct1 | |
| 15 | 8th Feb | 9:30am | Gloucester Ladies Jades | 32 | v | 33 | Cheltenham Ladies Phoenix | Leisure@ Ct1 | |
| 16 | 15th Feb | 2:00pm | Gloucester Ladies Jades | 37 | v | 32 | Steelers Embers | Leisure@ Ct1 | |
| 17 | 22nd Feb | 12:30pm | Gloucester Ladies Jades | 21 | v | 51 | Moody Blues | Leisure@ Ct1 | |
| 18 | 1st Mar | 11:00am | Gloucester Ladies Jades | 19 | v | 52 | Forest Foxes | Leisure@ Ct1 | |
| 19 | 8th Mar | 11:30am | Gloucester Ladies Jades | 21 | v | 41 | Seven Up B | High School Leckhampton | |
| 20 | 15th Mar | 11:30am | Gloucester Ladies Jades | 18 | v | 34 | Challengers Galaxy | High School Leckhampton | |
| 21 | 22nd Mar | 11:30am | Gloucester Ladies Jades | 28 | v | 38 | Gloucester City Queens Academy | High School Leckhampton | |
| 22 | 29th Mar | 2:00pm | Gloucester Ladies Jades | 33 | v | 36 | Cirencester Aqua | Leisure@ Ct1 |
The following adjustments were applied to Gloucester Ladies Jades: