| 1 | 21st Sep | 10:00am | Gloucester City Queens Academy | 36 | v | 34 | Moody Blues | High School Leckhampton | |
| 2 | 28th Sep | 2:00pm | Gloucester City Queens Academy | 33 | v | 28 | Forest Foxes | Leisure@ Ct1 | |
| 3 | 5th Oct | 9:30am | Gloucester City Queens Academy | 42 | v | 36 | Seven Up B | Leisure@ Ct1 | |
| 4 | 19th Oct | 11:00am | Gloucester City Queens Academy | 25 | v | 19 | Challengers Galaxy | Leisure@ Ct1 | |
| 5 | 26th Oct | 12:30pm | Gloucester City Queens Academy | 42 | v | 47 | Cheltenham Ladies Phoenix | Leisure@ Ct1 | |
| 6 | 16th Nov | 11:00am | Gloucester City Queens Academy | 53 | v | 29 | Cirencester Aqua | Leisure@ Ct1 | |
| 7 | 23rd Nov | 11:30am | Gloucester City Queens Academy | 18 | v | 40 | Hucclecote Tornadoes | High School Leckhampton | |
| 8 | 30th Nov | 11:00am | Gloucester City Queens Academy | 48 | v | 41 | Steelers Inferno | Leisure@ Ct1 | |
| 9 | 7th Dec | 10:00am | Gloucester City Queens Academy | 49 | v | 35 | Wildcats Panthers | High School Leckhampton | |
| 10 | 14th Dec | 11:30am | Gloucester City Queens Academy | 72 | v | 26 | Gloucester Ladies Jades | High School Leckhampton | |
| 11 | 11th Jan | 10:00am | Gloucester City Queens Academy | 35 | v | 31 | Steelers Embers | High School Leckhampton | |
| 12 | 18th Jan | 10:00am | Gloucester City Queens Academy | 42 | v | 49 | Moody Blues | High School Leckhampton | |
| 13 | 25th Jan | 2:00pm | Gloucester City Queens Academy | 30 | v | 32 | Forest Foxes | Leisure@ Ct1 | |
| 14 | 1st Feb | 9:30am | Gloucester City Queens Academy | 52 | v | 44 | Seven Up B | Leisure@ Ct1 | |
| 15 | 8th Feb | 11:00am | Gloucester City Queens Academy | 30 | v | 30 | Challengers Galaxy | Leisure@ Ct1 | |
| 16 | 15th Feb | 12:30pm | Gloucester City Queens Academy | 46 | v | 35 | Cheltenham Ladies Phoenix | Leisure@ Ct1 | |
| 17 | 22nd Feb | 11:00am | Gloucester City Queens Academy | 44 | v | 47 | Cirencester Aqua | Leisure@ Ct1 | |
| 18 | 1st Mar | 11:30am | Gloucester City Queens Academy | 31 | v | 38 | Hucclecote Tornadoes | High School Leckhampton | |
| 19 | 8th Mar | 11:00am | Gloucester City Queens Academy | 45 | v | 44 | Steelers Inferno | Leisure@ Ct1 | |
| 20 | 15th Mar | 10:00am | Gloucester City Queens Academy | 51 | v | 23 | Wildcats Panthers | High School Leckhampton | |
| 21 | 22nd Mar | 11:30am | Gloucester City Queens Academy | 38 | v | 28 | Gloucester Ladies Jades | High School Leckhampton | |
| 22 | 29th Mar | 10:00am | Gloucester City Queens Academy | 38 | v | 38 | Steelers Embers | Leisure@ Ct2 |
The following adjustments were applied to Gloucester City Queens Academy: