| 1 | 28th Sep | 3:30pm | Gloucester City Queens | 37 | v | 30 | Nomads Shadows | Barnwood Park | |
| 2 | 5th Oct | 2:00pm | Gloucester City Queens | 43 | v | 38 | United Hawks | Barnwood Park | |
| 3 | 19th Oct | 12:30pm | Gloucester City Queens | 48 | v | 46 | Lyndean | Barnwood Park | |
| 4 | 26th Oct | 11:00am | Gloucester City Queens | 51 | v | 27 | Fusion | Barnwood Park | |
| 5 | 9th Nov | 9:30am | Gloucester City Queens | 43 | v | 46 | Seven Up A | Barnwood Park | |
| 7 | 30th Nov | 9:30am | Gloucester City Queens | 31 | v | 46 | Cheltenham Ladies Griffins | Barnwood Park | |
| 8 | 7th Dec | 11:00am | Gloucester City Queens | 39 | v | 31 | Freestylers Eclipse | Barnwood Park | |
| 9 | 14th Dec | 12:30pm | Gloucester City Queens | 39 | v | 35 | Hucclecote Typhoons | Barnwood Park | |
| 10 | 11th Jan | 2:00pm | Gloucester City Queens | 28 | v | 59 | Stroud Pythons | Barnwood Park | |
| 11 | 18th Jan | 3:30pm | Gloucester City Queens | 31 | v | 35 | Churchdown Ladies Whitesox | Barnwood Park | |
| 12 | 1st Feb | 3:30pm | Gloucester City Queens | 40 | v | 36 | Nomads Shadows | Barnwood Park | |
| 13 | 8th Feb | 2:00pm | Gloucester City Queens | 32 | v | 31 | United Hawks | Barnwood Park | |
| 14 | 15th Feb | 12:30pm | Gloucester City Queens | 48 | v | 47 | Lyndean | Barnwood Park | |
| 15 | 22nd Feb | 11:00am | Gloucester City Queens | 41 | v | 40 | Fusion | Barnwood Park | |
| 16 | 1st Mar | 9:30am | Gloucester City Queens | 41 | v | 60 | Seven Up A | Barnwood Park | |
| 18 | 22nd Mar | 9:30am | Gloucester City Queens | 31 | v | 42 | Cheltenham Ladies Griffins | Plock Court | |
| 21 | 29th Mar | 11:00am | Gloucester City Queens | 32 | v | 32 | Stroud Pythons | St Peters | |
| 19 | 5th Apr | 11:00am | Gloucester City Queens | 41 | v | 34 | Freestylers Eclipse | Plock Court | |
| 20 | 12th Apr | 12:30pm | Gloucester City Queens | 23 | v | 32 | Hucclecote Typhoons | Plock Court | |
| 22 | 26th Apr | 3:30pm | Gloucester City Queens | 11 | v | 47 | Churchdown Ladies Whitesox | Plock Court |